5DM9
XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-05-18 |
| Detector | MPCCD |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.200, 79.200, 38.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.600 - 2.300 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vds |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.626 |
| Phasing software | REFMAC (5.8.0049) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.600 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 5774 | |
| <I/σ(I)> | 6.66 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 85 | 53.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 3 | 290 | PEG 6000, Sodium chloride, Sodium acetate |
