5DLY
Crystal structure of the plantazolicin methyltransferase BamL in complex with monoazolic desmethylPZN analog and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-04-04 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.700, 71.370, 48.790 |
| Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.23695 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.193 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 40012 |
| <I/σ(I)> | 15.6 |
| Completeness [%] | 96.1 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM Bis-Tris propane, pH 5.5, 200 mM Li2SO4, and 22-26% polyethylene glycol 3350 |
