5DLJ
Crystal Structure of Cas-DNA-N1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.894, 193.652, 193.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.835 - 2.600 |
R-factor | 0.1808 |
Rwork | 0.179 |
R-free | 0.20750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.050 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.088 | 0.040 | 0.807 |
Total number of observations | 499478 | ||
Number of reflections | 82019 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.9 | 99.7 | 99.8 |
Redundancy | 6.1 | 6.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 10% PEG400,50mM Na cacodylate,3mM spermine,15mM Magnesium chloride |