5DLF
Crystal Structure of Human DNA Polymerase Eta Inserting dATP Opposite O4-Methylhymidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.026, 99.026, 82.166 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.513 - 1.970 |
| R-factor | 0.1662 |
| Rwork | 0.164 |
| R-free | 0.21707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o3n |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.165 | 0.834 |
| Number of reflections | 32497 | |
| <I/σ(I)> | 14.298 | 2.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M MES pH 5.5, 5mM magnesium chloride, 18% PEG 2000 MME |
