5DKO
The structure of Escherichia coli ZapD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97888 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 108.900, 108.900, 106.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.525 - 2.400 |
| R-factor | 0.2511 |
| Rwork | 0.247 |
| R-free | 0.28810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oez |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.760 | |
| Number of reflections | 26464 | |
| <I/σ(I)> | 13.7 | 2.3 |
| Completeness [%] | 95.2 | 96.1 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15 mg/ml ZapD with 1.2 M ammonium sulfate, 100 mM HEPES, pH 7.5, 10 % PEG 600 |
