5DK2
Fc Heterodimer E356K/D399K + K392D/K409D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-08-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.857, 60.565, 159.448 |
| Unit cell angles | 90.00, 97.81, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.22825 |
| Rwork | 0.225 |
| R-free | 0.28866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.313 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 78.984 | 2.740 |
| High resolution limit [Å] | 2.600 | 8.220 | 2.600 |
| Rmerge | 0.031 | 0.609 | |
| Rmeas | 0.103 | ||
| Rpim | 0.055 | 0.023 | 0.440 |
| Total number of observations | 126900 | 3910 | 16418 |
| Number of reflections | 37058 | ||
| <I/σ(I)> | 11.1 | 29.1 | 1.8 |
| Completeness [%] | 97.2 | 92.2 | 95 |
| Redundancy | 3.4 | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM MES pH 6.5 + 12% PEG 8K + 200mM Sodium Acetate |
