5DJT
Crystal structure of LOV2 (C450A) domain in complex with Zdk2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | I 4 |
| Unit cell lengths | 110.140, 110.140, 37.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.507 - 1.400 |
| R-factor | 0.1471 |
| Rwork | 0.146 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WKQ and a homology model based on 2KZJ |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.343 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.065 | 0.360 |
| Number of reflections | 44465 | |
| <I/σ(I)> | 15.5 | 4.2 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 5.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M tri-sodium citrate pH 5.6, 0.2 M ammonium acetate, 32% (w/v) PEG 4000 and 0.1 M copper(II) chloride as an additive |
