5DJE

Crystal structure of the zuotin homology domain (ZHD) from yeast Zuo1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	60.480, 70.260, 95.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.675 - 1.850
R-factor	0.1765
Rwork	0.174
R-free	0.21970
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.241
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.100	1.490
Number of reflections	31477
<I/σ(I)>	11.93	1.21
Completeness [%]	99.7	99
Redundancy	6.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	protein at 15 mg/ml was dissolved in 100 mM NaCl, 5 mM HEPES, 5 mM DTT, pH 7.5. One microliter of protein solution was mixed with one microliter of reservoir solution, 30% PEG 3350, 30 mM MgCl2, 100 mM BisTris, pH 6.5, 5 mM DTT and equilibrated against the reservoir at 4C (277K)