5DJB
Structure of the Haliangium ochraceum BMC-H shell protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 3 |
| Unit cell lengths | 69.200, 69.200, 120.418 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.350 - 1.798 |
| R-factor | 0.1757 |
| Rwork | 0.170 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i6p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.350 | 1.860 |
| High resolution limit [Å] | 1.798 | 1.800 |
| Rmerge | 0.150 | 0.828 |
| Number of reflections | 118444 | |
| <I/σ(I)> | 10.3 | 2.2 |
| Completeness [%] | 99.4 | 94.6 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1 M ammonium tartrate, pH 6.5, 4% (v/v) formamide |
