5DJ3
Structure of the PLP-Dependent L-Arginine Hydroxylase MppP with D-Arginine Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.718, 108.277, 195.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.530 - 2.227 |
| R-factor | 0.1637 |
| Rwork | 0.162 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Holo-MppP (5DJ1) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 (705b) |
| Data scaling software | HKL-2000 (705b) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.530 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.106 | 0.726 |
| Number of reflections | 79816 | |
| <I/σ(I)> | 10 | 1.9 |
| Completeness [%] | 90.2 | 91.2 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% PEG 550 MMe, 50mM MgCl2, 100mM HEPES pH 8.0 |
