5DHF

Crystal Structure of hRio2 NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.479, 106.479, 303.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.718 - 2.285
R-factor	0.1791
Rwork	0.178
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.003
RMSD bond angle	0.617
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.320
High resolution limit [Å]	2.280	6.190	2.280
Rmerge	0.073	0.025	0.890
Rmeas	0.078	0.027	0.972
Rpim	0.028	0.010	0.376
Total number of observations	552057
Number of reflections	79060
<I/σ(I)>	9
Completeness [%]	98.6	93.4	99.8
Redundancy	7	6.6	5.9
CC(1/2)		0.997	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	17% PEG3350, 100mM Bis-Tris pH6.4, 200mM NH4NO3, 10mM Spermine HCl