5DHC
Cooperativity and Downstream Binding in RNA Replication
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2015-04-21 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9795 |
Spacegroup name | P 3 |
Unit cell lengths | 43.107, 43.107, 83.245 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.550 |
R-factor | 0.17164 |
Rwork | 0.170 |
R-free | 0.19501 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nfo |
RMSD bond length | 0.023 |
RMSD bond angle | 3.008 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.096 | 0.461 |
Number of reflections | 25146 | |
<I/σ(I)> | 17.54 | 2.14 |
Completeness [%] | 100.0 | |
Redundancy | 2.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 10% MPD, sodium cacodylate, spermine tetrahydrochloride, strontium chloride |