5DH9
Crystal Structure of PKI NES Flip Mutant Peptide in complex with CRM1-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-04-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.118, 106.118, 304.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.457 - 2.550 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hb2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.673 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 6.920 | 2.550 |
Rmerge | 0.087 | 0.025 | 0.987 |
Rmeas | 0.089 | 0.028 | |
Rpim | 0.041 | 0.012 | 0.465 |
Total number of observations | 320312 | ||
Number of reflections | 58218 | ||
<I/σ(I)> | 7.3 | ||
Completeness [%] | 99.6 | 96.7 | 100 |
Redundancy | 5.5 | 5 | 5.5 |
CC(1/2) | 0.998 | 0.669 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 17% PEG3350, 100mM Bis-Tris pH6.4, 200mM NH4NO3, 10mM Spermine HCl |