5DFY
Structure of the parental state of GAF3 from Slr1393 of Synechocystis sp. PCC6803 (in vitro assembled protein/chromophore)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00894 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.940, 62.940, 119.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.629 - 1.600 |
R-factor | 0.1559 |
Rwork | 0.155 |
R-free | 0.18060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.322 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.510 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 32428 | |
<I/σ(I)> | 27.87 | 6.44 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 12.71 | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 285 | 0.02 M sodium citrate pH 5.6, 0.1 M sodium chloride, 11 % (w/v) PEG 3350 |