5DFJ

Human APE1 E96Q/D210N mismatch substrate complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-20
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	44.462, 60.668, 73.161
Unit cell angles	82.78, 80.28, 89.20

Refinement procedure

Resolution	23.454 - 1.850
R-factor	0.1606
Rwork	0.159
R-free	0.19980
RMSD bond length	0.011
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Rmerge	0.132
<I/σ(I)>	12.2
Completeness [%]	100.0
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	10% PEG20000, 100 mM sodium citrate, 200 mM MgCl