5DF5
The structure of oxidized rat cytochrome c (T28E) at 1.30 angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-22 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.07812 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.609, 51.739, 61.748 |
| Unit cell angles | 110.01, 93.09, 91.88 |
Refinement procedure
| Resolution | 57.880 - 1.301 |
| R-factor | 0.14949 |
| Rwork | 0.148 |
| R-free | 0.17771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c0z |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.884 |
| Data reduction software | XDS (September 26, 2012) |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHENIX (1.8.1) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.881 | 1.335 |
| High resolution limit [Å] | 1.301 | 1.301 |
| Rmerge | 0.081 | 0.504 |
| Number of reflections | 90455 | |
| <I/σ(I)> | 15.3 | 2.6 |
| Completeness [%] | 96.6 | 89.38 |
| Redundancy | 5.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 20 mg/mL protein in water mixed 1:1 with 30% PEG 1500 |
