5DEG
Crystal structure of B*27:06 bound to the pVIPR peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.005 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.768, 81.684, 64.433 |
| Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.830 |
| R-factor | 0.17214 |
| Rwork | 0.171 |
| R-free | 0.19308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a83 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 43847 | |
| <I/σ(I)> | 34.7 | 6 |
| Completeness [%] | 99.1 | 92.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20%(w/v) PEG 8000, 100 mM Tris-HCl pH 7.0 |
