5DEC
Crystal structure of the small alarmone synthetase 1 from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.130, 77.690, 81.610 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 56.630 - 2.000 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vj7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.630 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.083 | 0.460 |
| Number of reflections | 64368 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 5.0 20% (w/v) PEG 6000 |
