5DE0
Dye-decolorizing protein from V. cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 |
Unit cell lengths | 77.700, 77.700, 156.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.296 - 2.240 |
R-factor | 0.2368 |
Rwork | 0.236 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qnr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.745 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.767 | |
Number of reflections | 50860 | |
<I/σ(I)> | 12.51 | 2.4 |
Completeness [%] | 99.8 | 99 |
Redundancy | 5.83 | 5.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | potassium thiocyanate, PEG 3000 |