5DCX
Structural studies of AAV2 Rep68 reveal a partially structured linker and compact domain conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2014-11-02 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.644, 178.706, 130.358 |
Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
Resolution | 29.814 - 2.600 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zo0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.781 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.810 | 29.810 | 2.690 |
High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
Rmerge | 0.075 | 0.038 | 0.311 |
Total number of observations | 43373 | 41772 | |
Number of reflections | 105074 | ||
<I/σ(I)> | 12 | 25.6 | 4.1 |
Completeness [%] | 99.1 | 96.4 | 98.9 |
Redundancy | 3.82 | 3.59 | 3.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM sodium cacodylate, pH 6.0, 90 mM potassium chloride, 20 mM magnesium chloride, 14-17% isopropanol |