5DCM
Structure of a lantibiotic response regulator: C-terminal domain of the nisin resistance regulator NsrR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.07145 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.380, 60.460, 56.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.850 - 1.600 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.21880 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.056 |
| Number of reflections | 124813 |
| <I/σ(I)> | 21.6 |
| Completeness [%] | 98.8 |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 0.1 M SPG buffer pH 8.0 and 25% (w/v) PEG 1500 |
