5DC0

CRYSTAL STRUCTURE OF MONOBODY GG3/ABL1 SH2 DOMAIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	131.437, 37.348, 39.705
Unit cell angles	90.00, 98.42, 90.00

Refinement procedure

Resolution	65.010 - 2.230
R-factor	0.1938
Rwork	0.191
R-free	0.24386
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.386
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	65.010
High resolution limit [Å]	2.230
Number of reflections	9392
<I/σ(I)>	17.4
Completeness [%]	98.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1M sodium tartrate pH=8 and 25% w/v polyethylene glycol 3350