5DC0
CRYSTAL STRUCTURE OF MONOBODY GG3/ABL1 SH2 DOMAIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.437, 37.348, 39.705 |
| Unit cell angles | 90.00, 98.42, 90.00 |
Refinement procedure
| Resolution | 65.010 - 2.230 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.24386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.386 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 65.010 |
| High resolution limit [Å] | 2.230 |
| Number of reflections | 9392 |
| <I/σ(I)> | 17.4 |
| Completeness [%] | 98.7 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M sodium tartrate pH=8 and 25% w/v polyethylene glycol 3350 |
