5DBO
Crystal structure of the tetrameric eIF2B-beta2-delta2 complex from C. thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.85506 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 109.430, 109.430, 219.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.948 - 3.000 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZEM and 4ZEO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.316 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 31063 | |
| <I/σ(I)> | 14.73 | 2.8 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 8.21 | |
| CC(1/2) | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | MES (pH 6.0), pentaerythritol propoxylate (5/4 PO/OH), NaCl |
