5DAG

Crystal structure of PZP domain of human AF10 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.283
Spacegroup name	P 41 21 2
Unit cell lengths	51.907, 51.907, 147.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.869 - 1.600
R-factor	0.192
Rwork	0.190
R-free	0.21430
RMSD bond length	0.007
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Rmerge	0.075
Number of reflections	22991
<I/σ(I)>	55
Completeness [%]	98.8
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Crystals were grown from a solution containing 0.1 M Tris-HCl pH 8.5, 25% PEG3350 using the hanging drop vapor diffusion method