5DA8
Crystal structure of chaperonin GroEL from
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 1 |
Unit cell lengths | 138.996, 159.781, 228.823 |
Unit cell angles | 75.46, 90.51, 91.22 |
Refinement procedure
Resolution | 48.600 - 3.000 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ki8 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.380 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.050 | 0.436 |
Number of reflections | 375234 | |
<I/σ(I)> | 10 | |
Completeness [%] | 99.0 | 98.5 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 0.1M Magnesium Chloride, 0.1M Imidazole, 35% MPD |