5DA1

A Dimerization-Dependent Mechanism Drives PRRSV NSP11 Functions As a Beta Interferon Antagonist and Endoribonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910
Spacegroup name	P 41 21 2
Unit cell lengths	77.317, 77.317, 204.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.750
Rwork	0.211
R-free	0.28190
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.320
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4-1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge		0.800
Number of reflections	17000
<I/σ(I)>	24.4	5.7
Completeness [%]	100.0	100
Redundancy	7.7	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium citrate tribasic dehydrate, PEG 3350