5D9H
Crystal structure of SPAK (STK39) dimer in the basal activity state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.386, 56.105, 99.958 |
Unit cell angles | 90.00, 108.28, 90.00 |
Refinement procedure
Resolution | 42.867 - 3.100 |
R-factor | 0.2403 |
Rwork | 0.236 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.181 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.900 | 50.000 | 3.200 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.069 | 0.047 | 0.492 |
Total number of observations | 38612 | ||
Number of reflections | 13658 | ||
<I/σ(I)> | 15.1 | 2.1 | |
Completeness [%] | 85.6 | 94.7 | 44.3 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 mM MES, 150 mM ammonium sulfate, 18% PEG4000, 1:1 drop ratio |