5D9A
Influenza C Virus RNA-dependent RNA Polymerase - Space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.280, 217.500, 597.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 4.300 |
R-factor | 0.31839 |
Rwork | 0.316 |
R-free | 0.36847 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d98 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.241 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.900 | 4.410 |
High resolution limit [Å] | 4.300 | 4.300 |
Rmerge | 0.204 | 0.993 |
Number of reflections | 95264 | |
<I/σ(I)> | 5.5 | 1.1 |
Completeness [%] | 99.0 | 93.1 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Drops were set up by mixing polymerase (5 mg/ml in 25 mM Hepes:NaOH, pH 7.5, 10% (v/v) glycerol, 0.5 M NaCl, 0.5 mM TCEP, 10 mM CaCl2) with 0.2 M NaCl, 0.1 M Na:HEPES, pH 7.5, 25% (w/v) PEG4000 and microseeds in a 2:1:1 protein:precipitant:seed ratio. |