5D91

Structure of a phosphatidylinositolphosphate (PIP) synthase from Renibacterium Salmoninarum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 2
Unit cell lengths	48.632, 94.069, 103.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.976 - 2.501
R-factor	0.2061
Rwork	0.204
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o6m
RMSD bond length	0.010
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	103.920	103.920	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.271	0.081	1.716
Rpim	0.063	0.022	0.467
Total number of observations	320975	7516	21993
Number of reflections	16926
<I/σ(I)>	9.9	31.9	1.7
Completeness [%]	98.2	99.9	84.5
Redundancy	19	16.9	13.6
CC(1/2)	0.999	0.999	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	20% (v/v) PEG 400, 0.1 M MES pH 6.7, 0.2 M lithium sulfate