5D8T
RNA octamer containing (S)-5' methyl, 2'-F U.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.91833 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 31.172, 31.172, 84.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.240 - 1.200 |
| R-factor | 0.121 |
| Rwork | 0.120 |
| R-free | 0.15700 |
| Structure solution method | SAD |
| RMSD bond length | 1.823 |
| RMSD bond angle | 42.196 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.145 | 0.584 |
| Number of reflections | 13758 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 14.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 40 mM sodium cacodylate, 20 mM hexamine Co(III) chloride, 20 mM MgCl2 |
