5D88
The Structure of the U32 Peptidase Mk0906
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-02 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 79.150, 79.150, 89.158 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.560 - 1.660 |
R-factor | 0.133 |
Rwork | 0.133 |
R-free | 0.15200 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.8) |
Refinement software | PHENIX (1.10-2131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.446 | |
Number of reflections | 73564 | |
<I/σ(I)> | 29.7 | 3.1 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 5.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.5 M NaOAc |