5D7S
Crystal structure of MOR04357, a neutralizing anti-human GM-CSF antibody Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.823, 66.922, 156.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.330 - 1.880 |
| R-factor | 0.21431 |
| Rwork | 0.213 |
| R-free | 0.25419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.303 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.330 | 2.000 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.088 | 0.618 |
| Number of reflections | 36714 | |
| <I/σ(I)> | 14.17 | |
| Completeness [%] | 97.8 | 96.4 |
| Redundancy | 7.9 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 293 | 26 % PEG3350, 0.2 M NaCl and 0.1 M Tris, pH 8.25, 10.5 mg/mL protein |
