5D7Q

Crystal structure of human Sirt2 in complex with ADPR and CHIC35

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-09-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	77.340, 78.170, 114.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.230 - 2.010
R-factor	0.21166
Rwork	0.211
R-free	0.22459
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d7o
RMSD bond length	0.007
RMSD bond angle	1.308
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.230	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.109	0.716
Number of reflections	46758
<I/σ(I)>	7.6
Completeness [%]	99.6
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.75	293	18 % (w/v) PEG 10000, 0.1 M Bis-Tris