5D7N
Crystal structure of human Sirt3 at an improved resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.990, 143.850, 89.460 |
| Unit cell angles | 90.00, 116.33, 90.00 |
Refinement procedure
| Resolution | 33.660 - 1.830 |
| R-factor | 0.17635 |
| Rwork | 0.175 |
| R-free | 0.20316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gls |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.336 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.660 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.084 | 0.815 |
| Number of reflections | 168813 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M magnesium chloride, 25 % (w/v) PEG 3350 |
