5D7E
Crystal structure of Taf14 YEATS domain in complex with H3K9ac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-06 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.164, 113.164, 26.524 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.000 - 1.900 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.220 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.520 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.660 |
| Number of reflections | 15763 | |
| <I/σ(I)> | 30.8 | 3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.2 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M sodium citrate and 48% PEG600 (v/v) |
