5D5Z

Structure of Chaetomium thermophilum Skn7 coiled-coil domain, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	47.004, 61.027, 75.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.2131
Rwork	0.209
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d5y
RMSD bond length	0.020
RMSD bond angle	1.981
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (2.7.029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.104	0.808
Number of reflections	24701
<I/σ(I)>	10.5	2.2
Completeness [%]	99.9	98.3
Redundancy	8.8	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	21 % PEG-6000