5D5Y

Structure of Chaetomium thermophilum Skn7 coiled-coil domain, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	24.823, 151.535, 47.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.030
R-factor	0.1664
Rwork	0.165
R-free	0.19900
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.853
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	SHELX
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.390	40.390	1.050
High resolution limit [Å]	1.030	5.630	1.030
Rmerge	0.066	0.066	1.103
Rpim	0.020	0.023	0.495
Total number of observations	514459	3491	9642
Number of reflections	45013
<I/σ(I)>	17.1	40.1	1.5
Completeness [%]	98.7	99.8	80.8
Redundancy	11.4	10.1	5.4
CC(1/2)	0.998	0.996	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20 % PEG-6000, 0.1 M HEPES-NaOH pH 7.0