5D5X

Crystal structure of Chaetomium thermophilum Skn7 with SSRE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.96500
Spacegroup name	C 1 2 1
Unit cell lengths	73.733, 43.328, 88.780
Unit cell angles	90.00, 92.78, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.2311
Rwork	0.228
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d5w
RMSD bond length	0.006
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.340	44.340	2.480
High resolution limit [Å]	2.390	8.940	2.390
Rmerge	0.092	0.051	0.793
Rpim	0.045	0.026	0.411
Total number of observations	56918	1046	4906
Number of reflections	11227
<I/σ(I)>	10.5	26.5	1.7
Completeness [%]	99.3	99.3	94
Redundancy	5.1	4.4	4.5
CC(1/2)	0.997	0.996	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25 % PEG-2000 MME, 0.1 M HEPES-NaOH pH 7.5