5D5G
Structure of colocasia esculenta agglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 122.012, 47.198, 82.251 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.986 - 1.740 |
| R-factor | 0.3455 |
| Rwork | 0.344 |
| R-free | 0.42720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r0e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.376 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 1.580 |
| High resolution limit [Å] | 1.536 | 1.536 |
| Rmerge | 0.380 | 0.610 |
| Number of reflections | 59836 | |
| <I/σ(I)> | 2.7 | 0.9 |
| Completeness [%] | 83.4 | 20 |
| Redundancy | 5.61 | 1.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | reservoir included 1.8 M ammonium sulphate and 0.1 M Hepes, pH 6.0; stock protein solution contained 8.1 mg/ml of lectin in 20 mM Tris pH 8.5; protein & reservoir soln mixed in 3:1 ratio at start and placed on siliconized cover slip |
