5D55
Crystal structure of the E. coli Hda pilus minor tip subunit, HdaB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.6531 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 112.294, 112.294, 61.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.400 - 2.000 |
| R-factor | 0.20735 |
| Rwork | 0.203 |
| R-free | 0.24831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4or1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.400 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.371 |
| Number of reflections | 26601 | |
| <I/σ(I)> | 9.3 | 2.7 |
| Completeness [%] | 98.1 | 95.1 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.2M Ammonium Citrate, 20% PEG 3350 |
