5D4Z
Crystal structure of Repressor from Salmonella-temperate phage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 |
Unit cell lengths | 61.599, 62.497, 267.896 |
Unit cell angles | 89.99, 89.97, 72.70 |
Refinement procedure
Resolution | 49.130 - 2.980 |
R-factor | 0.21855 |
Rwork | 0.216 |
R-free | 0.27324 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d50 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.254 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.980 |
Number of reflections | 75973 |
<I/σ(I)> | 22.8 |
Completeness [%] | 98.6 |
Redundancy | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 296 | 15%(w/v) PEG 4000, 0.1M magnesium sulfate |