5D4Y
A psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.386, 78.442, 88.724 |
| Unit cell angles | 90.00, 100.49, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.19584 |
| Rwork | 0.191 |
| R-free | 0.28480 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.580 |
| Data reduction software | Coot |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 24211 |
| <I/σ(I)> | 43.1 |
| Completeness [%] | 97.6 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 298 | 0.1M Tris, pH 8.5, 0.2M Li2SO4, 30% (w/v) PEG 4000 |
