5D49
Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978570 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.550, 74.250, 126.150 |
Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
Resolution | 37.020 - 1.990 |
R-factor | 0.1996 |
Rwork | 0.197 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jms |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.370 | 2.110 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.081 | 0.692 |
Number of reflections | 34690 | |
<I/σ(I)> | 8.99 | 1.26 |
Completeness [%] | 97.9 | 96.5 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 22-28% PEG 4000, 100-300 mM Lithium Sulfate and 100 mM Tris |