5D27
Crystal Structure of the P-Rex1 PH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-02-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 39.897, 39.897, 187.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.920 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d3v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.525 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 5.200 | 1.920 |
| Rmerge | 0.091 | 0.061 | 0.668 |
| Rmeas | 0.097 | 0.066 | 0.716 |
| Rpim | 0.035 | 0.024 | 0.255 |
| Total number of observations | 101710 | ||
| Number of reflections | 13243 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 97.9 | 95.1 | 96.9 |
| Redundancy | 7.7 | 7 | 7.7 |
| CC(1/2) | 0.996 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 294 | Bis Tris, Magnesium chloride, PEG 3350 |
