5D1Y
Low resolution crystal structure of human ribonucleotide reductase alpha6 hexamer in complex with dATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 356.010, 356.010, 356.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.902 - 9.005 |
| R-factor | 0.4074 |
| Rwork | 0.405 |
| R-free | 0.42580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hnc |
| RMSD bond length | 0.101 |
| RMSD bond angle | 1.512 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.861 | 80.000 | 10.100 |
| High resolution limit [Å] | 9.000 | 23.520 | 9.000 |
| Rmerge | 0.274 | 0.082 | 1.962 |
| Total number of observations | 50847 | ||
| Number of reflections | 6141 | ||
| <I/σ(I)> | 6.9 | 1.7 | |
| Completeness [%] | 99.8 | 98 | 100 |
| Redundancy | 15.7 | 13.2 | 16.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM HEPES, 32% v/v Jeffamine M600, 10 mM DTT |
