5D1K

Crystal Structure of UbcH5B in Complex with the RING-U5BR Fragment of AO7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-11-18
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	105.470, 45.658, 67.954
Unit cell angles	90.00, 118.09, 90.00

Refinement procedure

Resolution	33.339 - 1.780
R-factor	0.1707
Rwork	0.170
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2esk
RMSD bond length	0.007
RMSD bond angle	1.086
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.840
High resolution limit [Å]	1.780	3.830	1.780
Rmerge	0.057	0.028	0.467
Number of reflections	27367
<I/σ(I)>	11.8	69.124	3.013
Completeness [%]	99.1	99.9	92.7
Redundancy	7	7.3	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	20% PEG-10000, 8% ethylene glycol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K