5D1I

Structure of Cyclic nucleotide-binding-like protein from Brucella abortus bv. 1 str. 9-941

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	I 2 2 2
Unit cell lengths	56.096, 82.911, 103.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.682 - 2.000
R-factor	0.1877
Rwork	0.185
R-free	0.23320
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.728
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (dev_1819)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.085	0.054	0.381
Rmeas	0.087	0.055	0.388
Rpim	0.016	0.011	0.072
Total number of observations	478013
Number of reflections	16773
<I/σ(I)>	11.9
Completeness [%]	100.0	99.9	100
Redundancy	20	26.7	15
CC(1/2)		0.998	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	Potassium sodium tartrate tetrahydrate,olyethylene glycol 3350, KCl