5D0O

BamABCDE complex, outer membrane beta barrel assembly machinery entire complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	196
Detector technology	PIXEL
Collection date	2015-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.8233
Spacegroup name	P 41 21 2
Unit cell lengths	116.689, 116.689, 435.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.653 - 2.900
R-factor	0.2776
Rwork	0.276
R-free	0.30410
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.809
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.650	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.180
Number of reflections	67553
<I/σ(I)>	15
Completeness [%]	100.0	100
Redundancy	26.4	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4