5D0K

Structure of UbE2D2:RNF165:Ub complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 3
Unit cell lengths	113.046, 113.046, 135.667
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.699 - 2.650
R-factor	0.1943
Rwork	0.192
R-free	0.23770
RMSD bond length	0.014
RMSD bond angle	1.303
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.420
High resolution limit [Å]	2.650
Number of reflections	56144
<I/σ(I)>	12.7
Completeness [%]	99.7
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	MES, sodium chloride, PEG 6000