5D08
Crystal structure of selenomethionine-labeled epoxyqueuosine reductase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795, 0.9793, 0.9421 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.301, 95.780, 111.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.407 - 1.747 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.19580 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.954 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.747 | 3.770 | 1.750 |
| Rmerge | 0.084 | 0.047 | 0.578 |
| Total number of observations | 404248 | ||
| Number of reflections | 86822 | ||
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 94.8 | 92.5 | 93 |
| Redundancy | 4.7 | 4.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 0.1 M acetate (pH 4.5), 0.4 M (NH4)H2PO4, 12% (w/v) PEG 3350 |
